Coding
101-F1

Part:BBa_K2491002

Designed by: Philippe Hansen-Estruch   Group: iGEM17_CCA_San_Diego   (2017-10-01)


Part 1 of Fluorene Catabolic Pathway - 101

Composite part consisting of the upper fluorene catabolic pathway synthetic ORFs [flnB (1,1a-dihydroxy-1-hydro-9-fluorenone dehydrogenase), dbfA1 (angular dioxygenase large subunit), and dbfA2 (angular dioxygenase small subunit)] under the control of the constitutive promoter BBa_J23101 and RBS BBa_B0034 and carries the terminator BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 403
    Illegal BsaI.rc site found at 1515


[edit]
Categories
Parameters
None